Rabbit IgG Fc Rhodamine, TRITC

Product Overview

• Article Name: Rabbit IgG Fc Rhodamine, TRITC
• Biozol Catalog Number: ROC-011-0003
• Supplier Catalog Number: 011-0003
• Alternative Catalog Number: ROC-011-0003
• Manufacturer: Rockland Immunochemicals
• Category: Antikörper
• Conjugation: TRITC
• Alternative Names: Rabbit IgG F(c) fragment Rhodamine conjugation, TRITC conjugated Rabbit IgG F(c) fragment

Product Properties

• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Source: Rabbit
• Form: Lyophilized

Application Notes

• Application Dilute: IF Microscopy: 1:500 - 1:2,500
• Application Notes: Rabbit IgG F(c) fragment Rhodamine conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, uti

Images

SDS-PAGE of Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n 011-0002). Lane M: 3 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n 011-0002). Lane 2: Reduced Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n 011-0103). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n 011-0107). Load: 1 µg for F(ab) and F(c), 1.2 µg for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.