Rabbit IgM

Catalog Number: ROC-011-0107
Article Name: Rabbit IgM
Biozol Catalog Number: ROC-011-0107
Supplier Catalog Number: 011-0107
Alternative Catalog Number: ROC-011-0107
Manufacturer: Rockland Immunochemicals
Category: Antikörper
Application: SDS-PAGE
Conjugation: Unconjugated
Alternative Names: Rabbit immunoglobulin M
Concentration: 1.2 mg/mL by UV absorbance at 280 nm
Isotype: IgM
Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Source: Rabbit
Form: Liquid (sterile filtered)
Application Dilute: ELISA: User Optimized, IHC: User Optimized, IP: User Optimized, WB: User Optimized
Application Notes: Rabbit IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
SDS-PAGE of Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n 011-0002). Lane M: 3 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n 011-0002). Lane 2: Reduced Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n 011-0103). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n 011-0107). Load: 1 µg for F(ab) and F(c), 1.2 µg for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.