0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Source:
Rabbit
Form:
Lyophilized
Application Dilute:
ELISA: User Optimized, WB: User Optimized
Application Notes:
Rabbit IgG whole molecule Biotin conjugated can be utilized as a control reagent in both Western Blotting and ELISA format experiments. A streptavidin-conjugated reporter (ie. Streptavidin Peroxidase Conjugated) can be used for detection.
Identification and characterization of lung fibrocytes in IPF lungs.bRepresentative gating strategy for lung fibrocytes (CD45+CD15-CD3-CD19-CD56-CXCR4+Col-1+-cells) in explanted IPF lungs (n=3 bottom) and control lungs (HC, top) (n=4). Single cell suspensions were thawed and alive viable cells were further analyzed. Lung fibrocytes are present in the CD45+cell population. Contaminating and unwanted CD15+neutrophils, CD3+T cells, CD19+B cells and CD56+NK-cells were sequentially excluded. Isotype control for Col-1 was used to set the gate for Col-1+ cells.To control for non-specific labeling Rabbit IgG-Biotin conjugated (p/n 011-0602) was used. Fig. 2. PMID: 29747640.
SDS-PAGE of Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n 011-0002). Lane M: 3 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n 011-0002). Lane 2: Reduced Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n 011-0103). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n 011-0107). Load: 1 µg for F(ab) and F(c), 1.2 µg for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
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