rRat GM-CSF Protein

Product Overview

• Article Name: rRat GM-CSF Protein
• Biozol Catalog Number: ROC-012-001-V12-0020
• Supplier Catalog Number: 012-001-V12-0020
• Alternative Catalog Number: ROC-012-001-V12-0020
• Manufacturer: Rockland Immunochemicals
• Category: Proteine/Peptide
• Application: SDS-PAGE
• Conjugation: Unconjugated
• Alternative Names: Colony-stimulating factor (CSF), Pluripoietin-alpha, MGI1GM

Product Properties

• NCBI: 48750
• UniProt: P48750
• Buffer: See application note.
• Source: Rat
• Form: Lyophilized

Application Notes

• Application Notes: Granulocyte Macrophage Colony Stimulating Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Granulocyte Macrophage Colony Stimulating Factor in immunological assa

Images

SDS-PAGE of Rat Granulocyte Macrophage-Colony Stimulating Factor Recombinant Protein. Lane 1: 1 µg Rat GM-CSF in non-reducing conditions (-). Lane 2: Molecular weight marker. Lane 3: 1 µg Rat GM-CSF in reducing conditions (+). Lane 4: Molecular weight marker. Rat GM-CSF has a predicted MW of 14.5 kDa.

Bioactivity of Rat Granulocyte Macrophage-Colony Stimulating Factor Recombinant Protein. Serial dilutions of Rat GM-CSF, starting at 10 ng/mL, were added to FDCP-1 cells. After 91 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Rat GM-CSF is 0.9-1.3 pg/mL. This value is comparable to the typical expected range of less than 0.01 ng/mL.