Rat IgG Alkaline Phosphatase, ALP

Product Overview

• Article Name: Rat IgG Alkaline Phosphatase, ALP
• Biozol Catalog Number: ROC-012-0502
• Supplier Catalog Number: 012-0502
• Alternative Catalog Number: ROC-012-0502
• Manufacturer: Rockland Immunochemicals
• Category: Antikörper
• Conjugation: ALP
• Alternative Names: Rat IgG alkaline phosphatase conjugate, Rat IgG alk phos conjugate

Product Properties

• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Source: Rat
• Form: Liquid (sterile filtered)

Application Notes

• Application Notes: Rat IgG Alkaline Phosphatase Conjugated Whole Molecule Secondary Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Images

SDS-PAGE of Rat IgG F(c) Fragment Rhodamine Conjugated (p/n 012-0003). Lane M: 3 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rat IgG Whole Molecule (p/n 012-0102). Lane 2: Reduced Rat IgG F(c) Fragment Rhodamine Conjugated (p/n 012-0003). Lane 3: Reduced Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Reduced Rat IgM Whole Molecule (p/n 012-0107). Load: 1 µg of IgG, F(c), Fab, 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa, F(c) at 25 kDa, Fab at 25 kDa, IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.