Uricase Antibody, Goat, Polyclonal

Product Overview

• Article Name: Uricase Antibody, Goat, Polyclonal
• Biozol Catalog Number: ROC-100-101-092
• Supplier Catalog Number: 100-101-092
• Alternative Catalog Number: ROC-100-101-092
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: WB
• Species Reactivity: Bacteria
• Immunogen: Uricase [Bacillus species]
• Conjugation: Unconjugated
• Alternative Names: Uricase, ABC3735, goat anti-Uricase Antibody, goat anti-Uricase bacillus Antibody, urate oxidase (UO), factor-independent urate hydroxylase

Product Properties

• Clonality: Polyclonal
• Concentration: 90 mg/mL by Refractometry
• NCBI: 3202700
• UniProt: Q5WBJ3
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Bacillus
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Application Notes: Goat Anti-Uricase Antibody has been tested by western blot and suitable to be assayed against 1.0 ug of Uricase [Bacillus species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code 605-4302 and (ABTS (2,

Images

Rockland Goat anti Uricase antibody was used to detect purified Uricase under reducing and non-reducing conditions. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE and reduced samples of purified Uricase contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rockland Goat anti Uricase (200-101-092 lot 6732 1:5K in MB-070, ON 4 C). Primary antibody was detected with Rockland Dylight 649 conjugated Donkey anti Goat (605-743-125 1:10K 1.5 hr RT in MB-070 and imaged on the BioRad VersaDoc imaging system.