Sarcosine Oxidase Antibody, Goat, Polyclonal

Catalog Number: ROC-100-101-093
Article Name: Sarcosine Oxidase Antibody, Goat, Polyclonal
Biozol Catalog Number: ROC-100-101-093
Supplier Catalog Number: 100-101-093
Alternative Catalog Number: ROC-100-101-093
Manufacturer: Rockland Immunochemicals
Host: Goat
Category: Antikörper
Application: WB
Species Reactivity: Bacteria
Immunogen: Sarcosine Oxidase [Microbial]
Conjugation: Unconjugated
Alternative Names: Sarcosine oxidase subunit alpha, Sarcosine oxidase subunit, goat anti-Sarcosine Oxidase Antibody
Clonality: Polyclonal
Concentration: 90 mg/mL by Refractometry
NCBI: 10586229
UniProt: Q46337
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Lyophilized
Target: Microbial
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
Application Notes: Goat anti-Uricase Antibody has been tested by western blot an suitable to be assayed against 1.0 ug of Sarcosine Oxidase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code 605-4302 and (ABTS (
Rockland Goat anti Sarcosine Oxidase antibody was used to detect Sarcosine Oxidase. Samples of ~1 and 0.25µg of purified Sarcosine Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rockland Goat anti-Sarcosine Oxidase (p/n 200-101-093, lot 5951) at 1:5K in (p/n MB-070), ON at 4C. Primary antibody was detected with Rockland Dylight 649 conjugated Donkey anti-Goat (p/n 605-743-125) at 1:10K for 1.5 hr at RT in (p/n MB-070) and imaged on the BioRad VersaDoc imaging system.