Anti-Luciferase Antibody has been tested by western blot and is suitable in ELISA. This product can be assayed against 1.0 ug of Luciferase [Firefly] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code 611-1
Western Blot of Goat anti-Luciferase Antibody. Lane 1: Luciferase (Firefly). Load: 50 ng per lane. Primary antibody: Goat anti-Luciferase Antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP goat secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Luciferase.
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