Maltose Phosphorylase Antibody, Goat, Polyclonal

Product Overview

• Article Name: Maltose Phosphorylase Antibody, Goat, Polyclonal
• Biozol Catalog Number: ROC-100-101-230
• Supplier Catalog Number: 100-101-230
• Alternative Catalog Number: ROC-100-101-230
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: WB
• Species Reactivity: Bacteria
• Immunogen: Maltose Phosphorylase [E.coli]
• Conjugation: Unconjugated
• Alternative Names: goat anti-Maltose Phosphorylase Antibody, mapA antibody, mpA antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 90 mg/mL by Refractometry
• NCBI: 6233598
• UniProt: B2GEX2
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Escherichia coli
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Application Notes: Anti-Maltose Phosphorylase has been tested by western blot. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.

Images

Rockland Goat anti Maltose Phosphorylase antibody (200-101-230 lot 7850) was used to detect Maltose Phosphorylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System