Sucrose Phosphorylase Antibody, Goat, Polyclonal

Product Overview

• Article Name: Sucrose Phosphorylase Antibody, Goat, Polyclonal
• Biozol Catalog Number: ROC-100-101-231
• Supplier Catalog Number: 100-101-231
• Alternative Catalog Number: ROC-100-101-231
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: WB
• Species Reactivity: E. coli
• Immunogen: Sucrose Phosphorylase [E.coli]
• Conjugation: Unconjugated
• Alternative Names: goat anti-Sucrose Phosphorylase Antibody, Putative sucrose phosphorylase, Sucrose glucosyltransferase

Product Properties

• Clonality: Polyclonal
• Concentration: 90 mg/mL by Refractometry
• NCBI: 4492194
• UniProt: P76041
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Escherichia coli
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Application Notes: Anti-Sucrose phosphorylase has been tested by western blot and is suitable to be assayed against 1.0 ug of Sucrose Phosphorylase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code 605-4302 and (A

Images

Rockland Goat anti Sucrose Phosphorylase antibody (200-101-231 lot 7851) was used to detect purified Sucrose Phosphorylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System