Glucoamylase Antibody, Goat, Polyclonal

Product Overview

• Article Name: Glucoamylase Antibody, Goat, Polyclonal
• Biozol Catalog Number: ROC-100-101-234
• Supplier Catalog Number: 100-101-234
• Alternative Catalog Number: ROC-100-101-234
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: WB
• Species Reactivity: Other
• Immunogen: Glucoamylase [Rhizopus]
• Conjugation: Unconjugated
• Alternative Names: goat anti-Glucoamylase Antibody, 1 4 alpha D glucan glucohydrolase antibody, Amyloglucosidase antibody, Gluc 1 antibody, Gluc 2 antibody, Gluc 3 antibody, Glucan 1 4 alpha glucosidase antibody, Glucan 14 alpha glucosidase antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 90 mg/mL by Refractometry
• NCBI: 07683
• UniProt: P07683
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rhizopus
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
• Application Notes: Anti-Glucoamylase has been tested by western blot and is suitable to be assayed against 1.0 µg of Glucoamylase [Rhizopus] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code 605-4302 and (ABTS (2,2-azino-

Images

Rockland Goat anti Glucoamylase antibody (100-101-234 lot 7844) was used to detect purified glucoamylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT) . Images were collected using the BioRad VersaDoc System