0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Aspergillus niger
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
Application Notes:
Anti-glucose oxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Glucose Oxidase [Aspergillus niger] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG and (ABTS (2,2-azino-bis-[3
Western Blot of Goat anti Glucose Oxidase antibody. Lane 1: ~1ug Glucose Oxidase Reduced. Lane 2: ~0.25ug Glucose Oxidase Reduced. Lane 3: ~1ug Glucose Oxidase Non-Reduced. Lane 4: ~0.25ug Glucose Oxidase Non-Reduced. Primary antibody: Glucose Oxidase antibody at 1:5000 for overnight at 4C. Secondary antibody: Dylight 649 conjugated Donkey anti Goat (605-743-125 lot 20834 1:10K 1.5 hr RT. Block: MB-070 overnight at 4C. Predicted/Observed size: 65.6 kDa, 90 kDa for Glucose Oxidase. Other band(s): none.
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