Anti-Peroxidase (Goat) Antibody, Polyclonal

Product Overview

• Article Name: Anti-Peroxidase (Goat) Antibody, Polyclonal
• Biozol Catalog Number: ROC-100-1138
• Supplier Catalog Number: 100-1138
• Alternative Catalog Number: ROC-100-1138
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: WB
• Immunogen: Peroxidase [Horseradish]
• Conjugation: Unconjugated
• Alternative Names: HRP, Horseradish peroxidase, Goat anti-peroxidase antibody, anti-HRP

Product Properties

• Clonality: Polyclonal
• Concentration: 45.0 mg/mL by Refractometry
• NCBI: 42517
• UniProt: Q42517
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:9,000 - 1:40,000, WB: 1:900 - 1:4,000
• Application Notes: Anti-Peroxidase (Horseradish) (Goat) Antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Peroxidase [Horseradish] in a standard ELISA using Alkaline Phosphatase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit

Images

Western Blot of Goat anti-Peroxidase Antibody. Lane 1: horse radish peroxidase Reduced. Lane 2: horse radish peroxidase Reduced. Lane 3: horse radish peroxidase Non-Reduced. Lane 4: horse radish peroxidase Non-Reduced. Load: Lane 1 and 3 - 1.0 µg per lane, Lane 2 and 4 - 0.25 ug per lane. Primary antibody: Peroxidase antibody at 1:5,000 for overnight at 4C. Secondary antibody: DyLight(TM) 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 40 kDa/40 kDa for Horse radish peroxidase. Other band(s): Horse radish peroxidase splice variants and isoforms.