Protein A Antibody, Goat, Polyclonal

Product Overview

• Article Name: Protein A Antibody, Goat, Polyclonal
• Biozol Catalog Number: ROC-100-1177
• Supplier Catalog Number: 100-1177
• Alternative Catalog Number: ROC-100-1177
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: WB
• Immunogen: Protein A [Staphylococcus aureus]
• Conjugation: Unconjugated
• Alternative Names: Goat anti-Protein A Antibody, Goat anti Protein A

Product Properties

• Clonality: Polyclonal
• Concentration: 90 mg/mL by Refractometry
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
• Application Notes: Anti-Protein A has been tested by western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Images

Rockland Goat anti Protein A antibody (was used to detect Protein A under reducing and non-reducing conditions. Reduced samples of purified Protein A contained 4% BME and were boiled for 5 minutes. Samples of ~1ug and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Rockland Goat anti Protein A antibody (200-1177 lot 17938 1:5K in MB-0070, ON 4 C). Primary antibody was detected with Rockland Dylight 649 conjugated Donkey anti Goat 605-743-125 lot 20834 1:10K 1.5 hr RT in MB-070 and imaged on the BioRad VersaDoc imaging system.