ELISA: 1:5,000 - 1:25,000, Gel Shift: 0.5 to 1.0 µL, IHC: 1:500, WB: 1:500 - 1:3,000
Application Notes:
This product was tested by immunoblot and found to be reactive against NF-Y (B subunit specific) at a dilution of 1:2,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code 611-1302. Anti-NF-Y (B subunit
Immunohistochemistry. Rocklands Anti-NF-Y (B Subunit) antibody was diluted 1:500 to detect NF-YB in human thymus tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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