0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Saccharomyces cerevisiae
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
Application Notes:
Anti-Carboxypeptidase Y has been tested by western blot and is suitable to be assayed against 1.0 µg of Carboxypeptidase Y [Bakers Yeast] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code 611-1302 and (
Both the antiserum and IgG fractions of anti-Carboxypeptidase Y (Bakers Yeast) are shown to detect under reducing conditions of SDS-PAGE the 61,000 dalton enzyme in cellular extracts. Approximately 10 µg of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (611-1302) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.
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