Luciferase Antibody, Rabbit, Polyclonal

Catalog Number: ROC-100-401-137
Article Name: Luciferase Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-100-401-137
Supplier Catalog Number: 100-401-137
Alternative Catalog Number: ROC-100-401-137
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: DOT
Species Reactivity: Bacteria
Immunogen: Luciferase [Photobacterium fischerii]
Conjugation: Unconjugated
Alternative Names: rabbit anti-Luciferase Antibody, Alkanal monooxygenase alpha chain, Bacterial luciferase alpha chain, luxA, Alkanal monooxygenase beta chain, Bacterial luciferase beta chain, luxB
Clonality: Polyclonal
Concentration: 90 mg/mL by Refractometry
NCBI: 3280269
UniProt: P19907
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Lyophilized
Target: Photobacterium fischerii
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:5,000 - 1:25,000, IHC: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000
Application Notes: Luciferase Antibody has been tested by dot blot and is suitable for western blotting, immunohistochemistry, immunoprecipitation, and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Dot Blot results of Rabbit anti-Luciferase Antibody. Dots are Luciferase (Photobacterium fischerii) at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 60 min at RT. Primary Antibody: Rabbit anti-Luciferase at 1ug/mL 1hr RT. Secondary Antibody: Goat Anti-Rabbit IgG HRP 611-103-122 at 1:40,000 for 30min RT. Imaged with BioRad ChemiDoc, Chemi filter 5sec exposure.