Luciferase Antibody has been tested by dot blot and is suitable for western blotting, immunohistochemistry, immunoprecipitation, and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Dot Blot results of Rabbit anti-Luciferase Antibody. Dots are Luciferase (Photobacterium fischerii) at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 60 min at RT. Primary Antibody: Rabbit anti-Luciferase at 1ug/mL 1hr RT. Secondary Antibody: Goat Anti-Rabbit IgG HRP 611-103-122 at 1:40,000 for 30min RT. Imaged with BioRad ChemiDoc, Chemi filter 5sec exposure.
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