RFX5 (N-terminal specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-RFX5 Antibody, DNA binding protein RFX5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
Application Notes:
Anti-RFX5 has been tested in western blot. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 µl per assay.
Western Blot of Rabbit Anti-RFX5 antibody. Lane 1: nuclear extract lysates. Load: 10µg per lane. Primary antibody: RFX5 antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 65 kDa, 46 kDa for RFX5. Other band(s): none.
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