0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:25,000, Gel Shift: 0.5 to 1.0 uL per assay, WB: 1:500 - 1:1,000
Application Notes:
Anti-CREB-1 Antibody has been tested in western blot and suitable in ELISA assays. Specific conditions for reactivity should be optimized by the end user.
Anti-CREB is shown to detect CREB-1 present in Raji B cell nuclear extract. Detection occurs using a 1:1,000 dilution of antibody followed by a 1:5,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL. Film exposure was approximately 1. Other detection systems will yield similar results.
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