Anti-Notch antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid 2480-2510 of human Notch 1. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Anti-Notch-1 has been tested in IHC and ELISA. This antibody is useful in western blot. Specific conditions for reactivity should be optimized by the end user.
Rocklands anti-NOTCH 1 antibody was diluted 1:500 to detect NOTCH 1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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