This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 142-159 of Mouse GADD45 gamma. Swiss Prot accession number Q9Z111. This sequence represents the C-terminal end of the protein. Mouse and rat GADD45 g show 100% sequence homology. A single amino acid change is found in the human sequence (see below).
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-GADD45 gamma Antibody, Cytokine responsive protein CR6 antibody, DDIT2 antibody, GADD45G antibody, Growth arrest and DNA damage inducible gamma antibody, GRP17 antibody
This antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Reactivity in other immunoassays is unknown. This product was assayed by western blotting after immunoprecipitation of mouse GADD45 gamma from a lysate containing o
Immunoprecipitation and western blot using ROCKLAND Immunochemical Rabbit-anti-GADD45 gamma antibody. Lane 2 shows the detection of a band corresponding to mouse GADD45 gamma detected in a lysate over expressing the protein and the absence of staining in a similar lysate isolated from control cells (lane 1). Molecular weight markers (not shown) confirm a MW of ~19.2 kDa. For IP use ~10 µl of antiserum. For WB use a 1:500 dilution of antiserum. Detection occurs using a 1:2,000 dilution of HRP Goat-a-Rabbit IgG (611-103-122) with visualization via ECL. Film exposure approximately 45. Other detection systems will yield similar results.
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