Selenophosphate Synthetase 1 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Selenophosphate Synthetase 1 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-100-401-A22
• Supplier Catalog Number: 100-401-A22
• Alternative Catalog Number: ROC-100-401-A22
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Mouse
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS1.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-Selenophosphate Synthetase 1 Antibody, rabbit anti-SPS1 antibody, Selenide water dikinase 1, Selenophosphate synthetase 1, Selenium donor protein 1

Product Properties

• Clonality: Polyclonal
• Concentration: 85 mg/mL by Refractometry
• NCBI: 109079
• UniProt: Q8BH69
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:2,000
• Application Notes: This antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to SPS1 by western blotting in the appropriate cell lysa

Images

Western blot using Rocklands anti-SPS1 antibody shows detection of endogenous SPS1 in NIH3T3 cell (lane 1), mouse kidney, and liver tissue lysates (lanes 3-4). No signal is seen in NIH3T3 cells after pre-treatment with SPS1 siRNA (lane 2). SPS1 control (lane 5-6). Negligible cross-reactivity is seen against recombinant SPS2 (lane 7). The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.