PSCDBP antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region in the C-terminal portion of mouse Cybr.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-Cybr antibody, Cytohesin binder and regulator antibody, Cytohesin-binding protein HE antibody, Cytohesin-interacting protein antibody, Pleckstrin Homology Sec7 and Coiled-Coil Domains Binding Protein antibody, B31 antibody, PSCDBP antibody
This antibody has been tested for use in ELISA and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to Cybr by western blotting after IP in the appropria
Western blot using Rocklands anti-Cybr antibody shows detection of recombinant Cybr using anti-HA tag antibody after immunoprecipitation using anti-Cybr. Lane 5 contains lysate from control HEK293 cells. Lane 6 shows IP/WB of HEK293 cells expressing Cybr-HA. Lane 7 and 8 are loaded similarly to lanes 5 and 6 but were immunoprecipitated with anti-HA. Lane 9 contains marker. Lane 10 contains purified CybrHA. Personal Communication, V. Coppola, NCI, Bethesda, MD.
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