Cybr Antibody, Rabbit, Polyclonal

Catalog Number: ROC-100-401-A23
Article Name: Cybr Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-100-401-A23
Supplier Catalog Number: 100-401-A23
Alternative Catalog Number: ROC-100-401-A23
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: ELISA, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: PSCDBP antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region in the C-terminal portion of mouse Cybr.
Conjugation: Unconjugated
Alternative Names: rabbit anti-Cybr antibody, Cytohesin binder and regulator antibody, Cytohesin-binding protein HE antibody, Cytohesin-interacting protein antibody, Pleckstrin Homology Sec7 and Coiled-Coil Domains Binding Protein antibody, B31 antibody, PSCDBP antibody
Clonality: Polyclonal
Concentration: 85 mg/mL by Refractometry
NCBI: 227929
UniProt: Q91VY6
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Lyophilized
Target: Mouse
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:1,000 - 1:10,000
Application Notes: This antibody has been tested for use in ELISA and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to Cybr by western blotting after IP in the appropria
Western blot using Rocklands anti-Cybr antibody shows detection of recombinant Cybr using anti-HA tag antibody after immunoprecipitation using anti-Cybr. Lane 5 contains lysate from control HEK293 cells. Lane 6 shows IP/WB of HEK293 cells expressing Cybr-HA. Lane 7 and 8 are loaded similarly to lanes 5 and 6 but were immunoprecipitated with anti-HA. Lane 9 contains marker. Lane 10 contains purified CybrHA. Personal Communication, V. Coppola, NCI, Bethesda, MD.