Sars-Cov Nonstructural Protein 8 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Sars-Cov Nonstructural Protein 8 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-100-401-A53
• Supplier Catalog Number: 100-401-A53
• Alternative Catalog Number: ROC-100-401-A53
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: IF, WB
• Species Reactivity: Virus
• Immunogen: This whole rabbit serum was produced by repeated immunizations with a purified His- tagged recombinant protein corresponding to full-length SARS-Coronavirus nsp8.
• Conjugation: Unconjugated
• Alternative Names: ORF1a polyprotein, Replicase polyprotein 1a, nsp8, rabbit anti-Sars-Cov Nonstructural Protein 8 Antibody, sars-cov

Product Properties

• Clonality: Polyclonal
• Concentration: 85 mg/mL by Refractometry
• NCBI: 1489680
• UniProt: P0C6U8
• Buffer: None
• Form: Liquid (sterile filtered)
• Target: SARS
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: IF Microscopy: 1:300, IP: 1:60, WB: 1:1,000
• Application Notes: This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kDa in s

Images

Immunoprecipitation followed by western blotting using Rocklands Anti-nsp8 shows a predominant band at 21.8 kDa corresponding to full length SARS protein (panel A). Immunofluorescence Microscopy using anti-nsp8 6-h post infection of Vero-E6 cells (Panel B). For detection Cy3 conjugated Goat-anti-Rabbit IgG MX (611-104-122) was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.