Anti-CNP has been tested in western blot and IHC and is suitable for usage in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a 46kDa band by western blot corresponding to CNP protein in the appropriate cell lysate o
Western Blot of Rabbit Anti-CNP (2,3-cyclic nucleotide-3-phosphodiesterase) (Rabbit) Antibody. Lane 1: rat brain lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: CNP antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~ 46k, ~ 46k for CNP protein. Other band(s): none.
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