0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:200, IHC: 1:1000, IP: User Optimized, WB: 0.5-1:1000
Application Notes:
Anti-Aha1 Antibody has been tested in western blot, ICC, and IHC and is suitable for use in ELISA, IF microscopy and IP. Expect a band approximately ~38kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Rabbit anti-Aha1 antibody. Lane 1: A431 lysate. Lane 2: A549 lysate. Lane 3: HCT116 lysate. Lane 4: Hela lysate. Lane 5: Hek293 lysate. Lane 6: HepG2 lysate. Lane 7: HL-6D lysate. Lane 8: HUVEC lysate. Lane 9: Jurkat lysate. Lane 10: MCF7 lysate. Lane 11: PC3 lysate. Lane 12: T98G lysate. Lane 13: Rat Brain lysate. Load: 1ug per lane. Primary antibody: Aha1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti Rabbit IgG HRP at 1:10,000 for 30 min at RT. Block: 5% Blotto overnight at 4°C. Predicated/Observed size: 38.1 kDa/38.1kDa for Aha1.
* VAT and and shipping costs not included. Errors and price changes excepted