PDI Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: PDI Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-100-401-G29
• Supplier Catalog Number: 100-401-G29
• Alternative Catalog Number: ROC-100-401-G29
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Bovine, Canine, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rat, Sheep, Xenopus
• Immunogen: PDI Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against rat PDI synthetic peptide conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: PDA2, PDI, PDIA2, PDIP, pancreatic protein disulfide isomerase, Protein disulfide-isomerase, PDI, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta

Product Properties

• Clonality: Polyclonal
• Concentration: 85 mg/mL by Refractometry
• NCBI: 287164
• UniProt: P04785
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: IHC: User Optimized, IP: User Optimized, WB: 1:4000
• Application Notes: Anti-PDI Antibody is tested for use in WB, IP, IF microscopy and IHC. Expect a band approximately ~58kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of rabbit anti-PDI alpha antibody. Lane 1: Rat Brain. Lane 2: Rat Heart. Lane 3: Rat Kidney. Lane 4: Rat Liver. Lane: 5 Rat Lung. Lane 6:Rat Pancreas. Lane 7: Rat Skeletal Muscle. Lane 8:Rat Spleen. Lane 9: Rat Testes. Lane 10: Rat Thymus. Lane11: C2C12. Lane 12: NIH 3T3 Ctrl. Lane 13: Pam212. Primary antibody: PDI antibody at 1:1000 for overnight at 4C. Secondary antibody: Goat anti-rabbit IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4C. Predicted/Observed size: 56.9 kDa/58kD. Other band(s): none.