Rab4 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against a C-terminal region synthetic peptide from human Rab4.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
IHC: 1:100, WB: 1:1000-2000
Application Notes:
Anti-Rab4 Antibody is tested by WB, IHC, and IF microscopy. Expect a band approximately ~26kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of rabbit anti-Rab4 antibody. Lane 1: HeLa cell lysate. Primary antibody: Rab4 antibody at 1:1000 for overnight at 4C. Secondary antibody: Goat anti-rabbit IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4C. Predicted/Observed size:23.6 kDa/26kD. Other band(s): An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at ~75kDa and 50kDa in rat and mouse samples.
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