Anthrax Protective Antigen Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the internal region of the Anthrax PA protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid
Target:
Bacillus
Antibody Type:
Primary Antibody
Application Dilute:
WB: 1:1000
Application Notes:
Anti-Anthrax PA (Protective Antigen) Antibody has been tested in WB. Expect a band approximately 83kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Rabbit anti-Anthrax Protective Antigen antibody. Lane A: recombinant Anthrax PA protein. Lane B: recombinant Anthrax PA protein. Load: 10 µg per lane. Primary antibody: Anthrax Protective Antigen antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 83 kDa in lane B for Anthrax Protective Antigen. Lane A is pre-bleed as control. Other band(s): none.
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