Anti-Myosin-6 was prepared from whole rabbit serum produced by repeated immunizations with full length myosin-6 construct expressed in SF9 insect cells corresponding to porcine myosyn-6 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Swine
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000, WB: 1:1000
Application Notes:
Myosin-6 antibody has been tested by western blot. For western blots expect a band of approximately 150 kDa in size corresponding to full length and ~85kDa and ~60kDa for truncated myosin-6 protein. Specific conditions for reactivity should be optimized
Western Blot of Rabbit anti-Myosin-6 antibody. Lane 1: SF9 cell lysate of truncated smooth myosin. Lane 2: Jurkat lysate (p/n W09-001-370). Lane 3: LnCap lysate (p/n W09-001-GJ9). Lane 4: Recombinant myosin VI. Load: 20µg per lane for cell lysate. 50ng of recombinant protein. Primary antibody: Myosin 6 antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 150 kDa for Myo6.
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