TET2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a human TET-2 domain containing the N-terminal 156 aminoacids of the protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:50,000, WB: 1:5000
Application Notes:
Tet2 antibody has been tested by Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect band at ~230kDa and ~150kDa (isoforms). This antibody is suitable for use by ELISA.
Western Blot of Rabbit Anti-Tet2 Antibody. Lane 1: Whole Cell Extract. Lane 2: Cytosolic Extract. Lane 3: Nuclear Extract. Lane 4: Chromatin Fraction. Load: 35 µg per lane of HEK293T. Primary antibody: TET2 antibody at 1:5000 for 5 hours at room temperature. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~230 and ~150kDa. Other band(s): nonspecific.
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