TET3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a human TET-3 domain containing the N-terminal 160 amino acids of the protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:50,000, WB: 1:5000
Application Notes:
Tet3 antibody has been tested by Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect band at ~180kDa. This antibody is suitable for use by ELISA.
Western Blot of Rabbit Anti-TET3 Antibody. Lane 1: CEM nuclear extract. Lane 2: THP-1 nuclear extract. Load: 25 µg per lane. Primary antibody: TET3 antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: Isoforms of TET3 include 180kDa, 167kDa and 78kDa. Other band(s): nonspecific.
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