0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:20,000-1:50,000, WB: 1:1000-1:5000
Application Notes:
Anti-Morc3 Antibody is tested for use in Western Blot and suitable for ChIP and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 107.1 kDa in size corresponding Morc3 by western blotting in the appro
Western Blot of Rabbit anti-Morc3 antibody. Lane 1: Human embryonic stem cell. Lane 2: Human embryonic stem cell. Lane 3: C-Flag Mouse embryonic stem cell. Lane 4: C-Flag Mouse embryonic stem cell doxycycline induced. Load: 35 µg per lane. Primary antibody: hMorc3 antibody at 1:1000-1:5000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 107kDa/ ~170kDa. Other band(s): sumoylated Morc run higher.
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