ABCB8 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a internal portion of human ABCB8.
Conjugation:
Unconjugated
Alternative Names:
Rabbit anti-ABCB8 antibody, ATP-binding cassette sub-family B member 8 mitochondrial, Mitochondrial ATP-binding cassette 1, M-ABC1, ABCB-8, ABCB
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:20,000 - 1:50,000, WB: 1:250-1:500
Application Notes:
Anti-ABCB8 antibody has been tested by ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect band approximately 79.9 kDa in size corresponding to ABCB8 by western blotting in the appropriate cell lysat
Western Blot of Rabbit anti-ABCB8 antibody. Lane 1: Molecular weight marker. Lane 2: B8 crude membrane prep. Load: 10 µg per lane. Primary antibody: ABCB8 antibody at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for ABCB8.
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