MMTV was prepared from whole rabbit serum produced by repeated immunizations with a full length sequence for mouse mammary tumor virus capsid protein tagged with His.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000-1:20,000, WB: 1:1000-1:2000
Application Notes:
Anti-MMTV Antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect band approximately 26.7 kDa in size corresponding to MMTV capsid protein by western blotting in the appropriate
Western Blot of Rabbit anti-MMTV antibody. Lane 1: cell lysate negative control. Lane 2: cell lysate spiked with purified virus. Load: 10 µg per lane. Primary antibody: Mouse Mammary Tumor Virus Capsid antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~26.7kDa, ~28kDa and ~50kDa for MMTV. Other bands: higher bands are not unexpected since proteins are made from a larger precursor.
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