Anti-CBFb Antibody was produced in rabbits by repeated immunizations with human CBFb using two synthetic peptides containing sequences from the internal region of the protein.
Anti-CBFb Antibody has been tested in ChIP and ELISA. Specific conditions for reactivity should be optimized by the end user.
ChIP-seq results obtained with the antibody directed against CBFb. ChIP was performed as described in figure 1. The IPd DNA from 6 ChIPs were pooled and analyzed with an Illumina Genome Analyzer. Library preparation, cluster generation, and sequencing were performed according to the manufacturers instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3. Figure 3-5 shows three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
ChIP-seq results obtained with the antibody directed against CBFb. ChIP was performed as described in figure 1. The IPd DNA from 6 ChIPs were pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation, and sequencing were performed according to the manufacturers instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3. Figure 3-5 shows three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
Chromatin Immunoprecipitation results of Rabbit Anti-CBFb Antibody. Chromatin from 1.25 million formaldehyde cross-linked SKNO-1 cells was used with 4ul of Anti-human CBFb Antibody and 20ul of magnetic IgG beads per immunoprecipitation. QPCR was performed using primers specific for the FUT7, OGG1, NFE2, and SPI1 genes. ChIP results shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.
ELISA results of Rabbit anti-human CBFb antibody. Antigen: BSA conjugated CBFb. Coating amount: 0.1 µg per well. Dilution series: serial dilution. Estimated Antibody Titer to be 1:8,800. Substrate: TMB (p/n TMBE-1000).
ChIP-seq results obtained with the antibody directed against CBFb. ChIP was performed as described in figure 1. The IPd DNA from 6 ChIPs were pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation, and sequencing were performed according to the manufacturers instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3. Figure 3-5 shows three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
ChIP-seq results obtained with the antibody directed against CBFb. ChIP was performed as described in figure 1. The IPd DNA from 6 ChIPs were pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation, and sequencing were performed according to the manufacturers instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3. Figure 3-5 shows three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
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