Anti-hLeo1 Antibody was produced in rabbits by repeated immunizations with a full length recombinant protein of human Leo1 (component of the human PAF1/RNA polymerase ll complex).
Conjugation:
Unconjugated
Alternative Names:
RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein, RDL
Anti-hLeo1 Antibody has been tested in Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in the appropriate cell lysate or extract.
Western Blot results of Rabbit anti-hLeo1 antibody. Lane 1: HeLa nuclear extracts cells. Load: 20 µg. Primary antibody: hLeo1 antibody at 1:1000 for overnight at 4C. Secondary antibody: goat anti-rabbit HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBS-Tween overnight at 4C.
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