Peroxidase Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Peroxidase Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-100-4138
• Supplier Catalog Number: 100-4138
• Alternative Catalog Number: ROC-100-4138
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Species Reactivity: All
• Immunogen: Anti-Peroxidase antibody was produced by repeated immunization of rabbits with peroxidase isolated from horseradish.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-HRP, Peroxidase antibody, anti-HRP, Peroxidase antibody raised in rabbit, Rb-anti-HRP, Rb-anti-Peroxidase, Horseradish Peroxidase

Product Properties

• Clonality: Polyclonal
• Concentration: 90 mg/mL by Refractometry
• Buffer: See application note.
• Form: Lyophilized
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Peroxidase antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. Note the buffer for this anti-peroxidase antibody is 0.01 M Sodium Phosphate, 0

Images

Western Blot analysis of Rabbit Anti-Peroxidase Antibody. Lane 1: Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: HeLa Lysate/Peroxidase (5µg/0.1µg) [+]. Lane 3: HeLa Lysate/Peroxidase (5µg/0.05µg) [+]. Lane 4: HeLa Lysate/Peroxidase (5µg/0.01µg) [+]. Lane 5: HeLa Lysate (p/n W09-000-364) (5µg) [-]. Primary Antibody: Anti-Peroxidate at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP mx10 (p/n 611-103-122) at 1:70,000 for 30 mins at RT.