NFkB cRel Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: NFkB cRel Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-100-4166
• Supplier Catalog Number: 100-4166
• Alternative Catalog Number: ROC-100-4166
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: NFkB cRel peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-cRel Antibody, rabbit anti-NFkB Antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, REL, c-Rel

Product Properties

• Clonality: Polyclonal
• Concentration: 80 mg/ml by Refractometry
• NCBI: 5966
• UniProt: Q04864
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:25,000, Gel Shift: 1:500, WB: 1:500 - 1:3,000
• Application Notes: Anti-NFkB cRel was tested by ELISA and by immunoblot. Anti-NFkB cRel is suitable for the detection by immunoblot of human NFkB cRel at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) cod

Images

Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.