IKB alpha Antibody was produced by repeated immunizations with a synthetic IkBa peptide corresponding to a region near the C-terminus of the human protein.
Anti-IkBa Antibody has been tested by western blot and is suitable for ELISA, and IHC. Researchers should determine optimal titers for applications that are not stated below.
Western Blot of Rabbit anti-IKBalpha C-terminal antibody. Lane 1: IKBalpha C-terminal. Load: 50 ng per lane. Primary antibody: IKBalpha C-terminal antibody at 1:500 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min RT. Predicted/Observed size: 61 kDa, 61 kDa for IKBalpha C-terminal.
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