Ribonuclease A Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Ribonuclease A Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-100-4188
• Supplier Catalog Number: 100-4188
• Alternative Catalog Number: ROC-100-4188
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Species Reactivity: Bovine
• Immunogen: Anti-Ribonuclease A was produced by repeated immunizations with bovine pancreatic Ribonuclease A.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-Ribonuclease A Antibody, Ribonuclease pancreatic, RNase 1, RNase A, RNS1

Product Properties

• Clonality: Polyclonal
• Concentration: 90 mg/mL by Refractometry
• NCBI: 282340
• UniProt: P61823
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Bovine
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:30,000 - 1:150,000, IP: 1:100, WB: 1:3,000 - 1:15,000
• Application Notes: Anti-Ribonuclease A Antibody is suitable for western blotting, IP and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Images

Rab27A deficiency caused increased expression of Rab27B, but did not affect its activity.(A and B) Examples of total lysates of isolated pancreatic acinar cells from wild-type C3H/HeSnJ or ashen mice were analyzed by western blot. Each lane represents samples from one mouse. (B) Densitometry analysis on the western blot results from all samples run as in (A). The results are mean SE from five mice of each genotype. *P < 0.05. (C) Active form of Rab27B and Rab3D at basal level in isolated acini was examined by GST-SHD and GST-Rim pulldown, respectively. Pulldown fractions were analyzed by western blot. This experiment was repeated three times with similar results. (D) The expression of major digestive enzymes (amylase, chymotrypsin, lipase, and elastase) and other Rab proteins (Rab6 and Rab11) was also not changed in western blots on lysates from isolated ashen mouse acinar cells. (E) The expression of major digestive enzymes (amylase, chymotrypsin, elastase, carboxypeptidase A and ribonuclease A) was also not changed in western blots of purified ashen mouse zymogen granules. Figure provided by CiteAb. Source: PLoS One, PMID: 25951179.