Anti-B-Phycoerythrin has been tested by western blot and is suitable for dot blot, ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.
Western Blot of Rabbit anti-B-Phycoerythrin antibody. Lane 1: B-Phycoerythrin reduced. Lane 2: B-Phycoerythrin reduced. Lane 3: none. Load: ~1µg per lane. Primary antibody: ß-Phycoerythrin antibody at 1:1000 for overnight at 4C. Secondary antibody: Dylight 649 conjugated Donkey anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: MB-070 overnight at 4C. Predicted/Observed size: 18.5 kDa, ~25 kDa for B-Phycoerythrin. Other band(s): none.
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