Malate Dehydrogenase Antibody, Sheep, Polyclonal

Product Overview

• Article Name: Malate Dehydrogenase Antibody, Sheep, Polyclonal
• Biozol Catalog Number: ROC-100-601-145
• Supplier Catalog Number: 100-601-145
• Alternative Catalog Number: ROC-100-601-145
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Species Reactivity: Porcine
• Immunogen: Malate Dehydrogenase [Pig Heart]
• Conjugation: Unconjugated
• Alternative Names: sheep anti-Malate Dehydrogenase Antibody, Malate dehydrogenase cytoplasmic, Cytosolic malate dehydrogenase

Product Properties

• Clonality: Polyclonal
• Concentration: 80 mg/mL by Refractometry
• NCBI: 396894
• UniProt: P11708
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Swine
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Application Notes: This product has been assayed against 1.0 ug of Malate Dehydrogenase [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code 611-1302 and (ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]

Images

Western Blot using Anti-Malate Dehydrogenase (Pig Heart) (SHEEP) Antibody. Two different molecular weight groups of isoforms were detected for MDH1 at about 36 kDa using Anti-MDH1 (pig heart) 100-601-145 at 1:1500, Fluorescent Cy-5 labelled secondary at 1:500 and for GDH at about 60 kDa using Anti-GDH (bovine liver) 100-4158 at 1:1500, anti-rabbit secondary at 1:500. Light intensities revealed unchanged amount of total soluble MDH1 but increased amount of total soluble GDH in AD when compared to controls. Values of light intensities are given as mean S.E.M. *p 0.05. Fig. 6. PMID: 16298240.