Lactoperoxidase Antibody, Sheep, Polyclonal

Product Overview

• Article Name: Lactoperoxidase Antibody, Sheep, Polyclonal
• Biozol Catalog Number: ROC-100-601-146
• Supplier Catalog Number: 100-601-146
• Alternative Catalog Number: ROC-100-601-146
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: WB
• Species Reactivity: Bovine
• Immunogen: Lactoperoxidase [Bovine Milk]
• Conjugation: Unconjugated
• Alternative Names: sheep anti-Lactoperoxidase Antibody, Lactoperoxidase, LPO

Product Properties

• Clonality: Polyclonal
• Concentration: 90 mg/mL by Refractometry
• NCBI: 280844
• UniProt: P80025
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Bovine
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:18,000 - 1:75,000, IP: 1:100, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Lactoperoxidase has been tested in western blot and is suitable in ELISA and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.

Images

Rockland Sheep anti Lactoperoxidase antibody (200-601-146 lot 5243) was used to detect Lactoperoxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated secondary antibody (613-741-168 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System