IL-1 Receptor Type II Antibody, Rabbit, Polyclonal
Biozol Catalog Number:
ROC-109-401-305S
Supplier Catalog Number:
109-401-305S
Alternative Catalog Number:
ROC-109-401-305S
Manufacturer:
Rockland Immunochemicals
Host:
Rabbit
Category:
Antikörper
Application:
WB
Species Reactivity:
Human, Primate
Immunogen:
This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-1R type II.
Conjugation:
Unconjugated
Alternative Names:
IL1RB antibody, Interleukin 1 receptor type II precursor antibody, MGC47725 antibody, p68 antibody, Type II interleukin 1 receptor beta antibody, Antigen CDw121b antibody, rabbit anti-interleukin-1 Receptor Type II Antibody
This antiserum against Human IL-1 Receptor Type II (IL-1RII) has been tested by western blot and is suitable for use in ELISA, radioimmunoassays and immunoprecipitation. Reactivity in other immunoassays is unknown. IL-1RII has been termed p68 or CD121 in
Western blot using Rocklands anti-IL-1RII antibody. This blot shows detection of an IL-1RII-GST fusion protein (~500 ng, lane 1, green, 800 nm channel). Minimal reactivity is observed against GST (data not shown). Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was for 2 hrs at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (611-132-122) for 45 min at room temperature. Molecular weight markers are shown for size comparison (lane M, red, 700 nm channel). IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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