Anti-NEDD1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the 343-667 region of human Nedd1.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:100,000, WB: 1:10,000
Application Notes:
This antiserum has been tested for use in ELISA and western blotting using a recombinant truncated Nedd1 protein. Specific conditions for reactivity and detection of Nedd1 should be optimized by the end user. Expect a band approximately ~73 kDa in size c
Anti-NEDD1 in Western Blot using Rockland Immunochemicals Anti-NEDD1 Antibody shows detection of a 73 kDa band corresponding to endogenous NEDD1 in lysates of S phase HeLa cells silenced for either control Luciferase or NEDD1. In right lane (NEDD1i): lysates from sh-NEDD1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-NEDD1 Antibody was used at 1:10,000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.
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