Anti-Goat IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
Clonality:
Monoclonal
Concentration:
1000X (see application note, titrated reagent enough for 80 blots)
Clone Designation:
[eB270]
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Goat
Application Dilute:
WB: 1:1000
Application Notes:
Goat IgG HRP TrueBlot has been tested in western blot and immunoprecipitation and may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Goat IgG TrueBlot is provided as 1000X solution. To conserve reagent, we re
Goat TrueBlot IP / Western Blot: Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with goat anti-human Stat1 and immunoprecipitated using Protein G. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti-Stat1 using Goat TrueBlot ULTRA: Anti-Goat IgG HRP (lane 1) and conventional HRP-conjugated anti-goat polyclonal antibody (lane 2).
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