Anti-Sheep IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
Clonality:
Monoclonal
Concentration:
1000X (see application note, titrated reagent enough for 80 blots)
Clone Designation:
[eB270]
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Sheep
Application Dilute:
WB: 1:1000
Application Notes:
Sheep IgG HRP TrueBlot has been tested in western blot and immunoprecipitation and may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Sheep IgG TrueBlot is provided as 1000X solution. To conserve reagent, we
Sheep TrueBlot IP / Western Blot: Jurkat cell lysate (500 µg) was incubated with 2 µg of sheep anti-SLP76 and immunoprecipitated using Protein G. 20 µg Jurkat lysate (lane 1), immunoprecipitate (lane 2) and 1.0 µg and 0.1.0 µg reduced anti-SLP76 (lanes 3 and 4) were subjected to electrophoresis, transferred to a PVDF membrane, and immunoblotted with anti-SLP76 using conventional HRP-conjugated anti-sheep polyclonal antibody (left panel) and Sheep TrueBlot ULTRA: Anti-Sheep IgG HRP (right panel).
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