Uricase Antibody, Goat, Polyclonal

Product Overview

• Article Name: Uricase Antibody, Goat, Polyclonal
• Biozol Catalog Number: ROC-200-101-092-0100
• Supplier Catalog Number: 200-101-092-0100
• Alternative Catalog Number: ROC-200-101-092-0100
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: WB
• Species Reactivity: Bacteria
• Immunogen: Uricase [Bacillus species]
• Conjugation: Unconjugated
• Alternative Names: goat anti-uricase antibody, Urate oxidase antibody, Factor-independent urate hydroxylase

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 3202700
• UniProt: Q5WBJ3
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Bacillus
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:1,000 - 1:2,500, IP: 1:100, WB: 1:500 - 1:1,000
• Application Notes: Uricase Antibody has been tested by western blot and is assayed against 1.0 ug of Uricase [Bacillus species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code 611-1302 and (ABTS (2,2-azino-bis-[3-ethylben

Images

Rockland Goat anti Uricase antibody was used to detect purified Uricase under reducing and non-reducing conditions. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE and reduced samples of purified Uricase contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rockland Goat anti Uricase (200-101-092 lot 6732 1:5K in MB-0070, ON 4 C). Primary antibody was detected with Rockland Dylight 649 conjugated Donkey anti Goat (605-743-125 1:10K 1.5 hr RT in MB-070 and imaged on the BioRad VersaDoc imaging system.