0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Candida
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
Application Notes:
Anti-Invertase Antibody has been tested by western blot. This product is suitable by assay against 1.0 ug of Invertase [Candida] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code 611-1302 and (ABTS (2,2-a
Rockland Goat anti Invertase antibody (100-101-189 lot 6736) was used to detect purified invertase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT) . Images were collected using the BioRad VersaDoc System
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